Synchrotron Radiation and StructuralBiology

Synchrotron Radiation and StructuralBiology

-

生命科学専攻・生体物質構造解析学・選択科目

Graduate School of Science

1Year

HSSML5MCA1

2.00Credits

講義 (Lecture)

Spring semester 2026

(Spring semester)

清水　伸隆

非常勤講師

Japanese

9

15 minutes after class, lecture room

nobutaka.shimizu.uj@riken.jp

ー

1◎／6〇

N/a

ー

Purpose of lecture
This lecture will introduce synchrotron radiation (SR) structural biology, which is a primary method for analyzing the three-dimensional structure of proteins in modern life science research. It will cover the basics of SR beamline technology, the latest technology, and analysis methods for protein X-ray crystallography and Bio-Small Angle X-ray Scattering to deepen understanding of state-of-the-art SR structural biology and its application development.
Objectives
To deepen knowledge of three-dimensional structures of proteins in the life sciences and their analytical methods and to acquire the ability to apply a structural biology perspective to research in graduate school.

Keywords: X-ray crystallography, Bio-Small Angle X-ray Scattering(SAXS), synchrotron structural biology

Three-dimensional structures of biological macromolecules, i.e., Proteins, are essential information to understand biological functions. Most of the 3D structures are analyzed by Synchrotron Radiation (SR) Macromolecular Crystallography. I give lectures on the basis and features of SR to SR Structural Biology with introductions of current topics.

Class plan / closely-packed series of lectures
１. Introduction of Synchrotron Radiation (SR) Structural Biology.
２. Methods and technologies in SR Macromolecular Crystallography.
３. Topics 1 of Current SR Macromolecular Crystallography (High-throughput etc.).
４. Topics 2 of Current SR Macromolecular Crystallography (Microcrystal etc.).
５. Topics 3 of Current SR Macromolecular Crystallography (Radiation damage etc.).
６. Features objects and prospects of SR Structural Biology.

In-person

・対面授業のみ。
・遠隔授業単位上限の適用を受けない。

Fully permitted

生成ＡＩの利用にあたっては『本学の教育における生成ＡＩの取扱いについて（学生向け）』の記載内容について留意すること。この授業においては、授業内、予習復習、レポート等を含む成果物作成等において生成ＡＩの利用を全面的に許可しており、生成ＡＩの利用について制限を設けないが、生成ＡＩによる出力結果をそのまま課題・レポートとして提出してはならない。生成ＡＩの出力した内容について、事実関係の確認や出典・参考文献を確認・追記することが重要である。使用した場合にその旨をレポート等に記載するかどうか等については、担当教員の指示に従うこと。

Distribute lecture materials from Universal Passport as appropriate.

"BIOMOLECULAR CRYSTALLOGRAPHY" Bernhard Rupp (2009) Garland Science
Other references will be provided in class.

Before the class, students must study the lecture materials distributed in advance. （30ｈ）
After the lecture, students must confirm any questions they may have as a post-lecture study and check with the lecturer in charge, as necessary. （30ｈ）

Not supported

Grading Criteria
Students will be given a grade of S (90 points or more), A (80 points or more), B (70 points or more), or C (60 points or more) based on the level of achievement of the abilities described in the lecture objectives and achievement goals, and will receive credit.
Grading Method
The evaluation will be based on reports and overall assessment, including the student's attitude (active questioning, etc.).

Comment and discuss as needed.

It is to be desired that the participant has studied the basics of crystallography.

該当しない

The lecturer will be replaced by Nobutaka Shimizu from 2025.