Analysis of Biosupramolecular Structure

Course Title

Course Title in English

Analysis of Biosupramolecular Structure

Course Type

-

生命科学専攻・生体物質構造解析学・選択科目

Eligible Students

Graduate School of Science

Target Grade

1Year

Course Numbering Code

HSSML5MCA1

Credits

2.00Credits

The course numbering code represents the faculty managing the subject, the department of the target students, and the education category (liberal arts / specialized course). For detailed information, please download the separate manual from the upper right 'question mark'.

Type of Class

講義 (Lecture)

Eligible Year/Semester

Fall semester 2026

Instructor

村本和優

Affiliation

理学研究科

Language of Instruction

Japanese

Related SDGs

9

Office Hours and Location

随時・研究2期棟S206号室

Contact

ユニパの授業Q&Aにより事前連絡

Corresponding Diploma Policy

A double circle indicates the most relevant DP number and a circle indicates the associated DP.

Corresponding Undergraduate School DP

ー

Corresponding Graduate School DP

1◎／6〇

Corresponding University-Wide DP

N/a

Academic Goals of Teacher Training Course

ー

Course Objectives and Learning Outcome

This course aims at understanding structure and function of biosupramolecule and methods of structural analysis.

Subtitle and Keywords of the Class

サブタイトル：　エネルギー変換系膜タンパク質複合体構造解析
キーワード：　呼吸鎖タンパク質複合体構造、X線結晶解析、構造機能相関

Course Overview and Schedule

Lecture about structural examples of bioenergetic membrane protein complexes with their functions, X-ray structural analysis and property of structures.
1. Membrane protein complexes in the energy transducing system
2. Protein expression, purification and crystallization
3. Structural analysis (Optical microscopy, Electron microscopy, X-ray crystallography)
4. X-ray crystallography (Diffraction, Wave interference)
5. X-ray crystallography (X-ray diffraction, Structure factor)
6. X-ray crystallography (Crystal structure factor)
7. X-ray crystallography (Phase determination)
8. X-ray crystallography (Electron density, Structural refinement)
9. Property of structures (Molecular surface, Electrostatic interaction, Analysis of pKa)
10. Property of structures (Hydrogen bond, Metal atoms, Solvent and lipid molecules, Structural changes)
11. Systems composed of biosupramolecule (ATP synthesis)
12. Systems composed of biosupramolecule (Bacterial motility).
13. Structure and function of biosupramolecule (Pumps, Active transporters)
14. Structure and function of biosupramolecule (Channels, Transporters)
15. Conclusion

In-person/Remote Classification

In-person

Implementation Method and Remote Credit Limit Application

対面授業のみ（パソコン、大画面モニターを使用する。プリントを配布する。）
遠隔授業単位上限の適用を受けない

Uses of Generative AI

Limited permission for use

Precautions for using Generative AI

生成ＡＩの利用にあたっては『本学の教育における生成ＡＩの取扱いについて（学生向け）』の記載内容について留意すること。課題学習の作品、レポートの作成には生成AI の利用不可。

Textbook

Prints offered in this class

References

「生体高分子結晶のX線構造解析」(2022) 竹中章郎他（丸善出版）
「タンパク質のX線結晶解析法　第2版」(2008) J. Drenth (シュプリンガー・ジャパン)
「クライオ電子顕微鏡ハンドブック」(2023) 難波啓一他（NTS）
“Bioenergetics 4” (2013) D. G. Nicholls and S. J. Ferguson (Academic Press)
「タンパク質の構造入門　第2版」(2000) C. Branden & J. Tooze (Newton Press)

Contents and Estimated Time for Pre- and Post- Learning (Preparation and Review)

【事前学習】各回の授業計画と関連する参考⽂献中の内容を予習する。（20h）
【事後学習】各回の授業内容を復習し、指⽰された課題に取り組む。（40h）

Contents of Active Learning

採⽤しない

Grading Criteria and Methods

Report

How to Disclose Assignments and Exam Results

課題については、授業時もしくはユニバーサルパスポートでコメントする。

Precautions and Requirements for Course Registration

Practical Education

該当しない

Remarks

In cases where any differences arise between the English version and the original Japanese version, the Japanese version shall prevail as the official authoritative version.

Syllabus data