Cell biology 6

Cell biology 6

Major Courses／Teacher training courses

-

School of Science

3Year

HSSBA3MCA1

2.00Credits

講義 (Lecture)

Spring semester 2026

(Spring semester)

吉田　秀郎

Faculty of Science

Japanese

英語も少し使用します。

9

Anytime at Rm 507

hide@sci.u-hyogo.ac.jp

2◎／4◎／11◎

ー

1-1◎／1-2〇

Ability to keep polishing

Course Objectives：In this lecture, students will learn the most advanced knowledge of cell biology, "the life of proteins (proteostasis)," and acquire the ability to propose new research themes by themselves by making questions for the lecture after each lecture. The abilities thus acquired are essential for future executive positions in pharmaceutical, food, chemical, and other companies.
Aim：(1) To be able to give an overview of the state-of-the-art in cell biology (molecular chaperones and ubiquitin-proteasome system, autophagy, subcellular localization mechanism of proteins). (2) To be able to propose original questions in light of the current state of the art in cell biology.

【Key words】
Molecular chaperones in archaea, eubacteria and eukaryotes、folding、heat shock proteins、cytosolic chaperone、ER chaperone、mitochondrial chaperone、chaperones and human disesases、amyloid、prion、Alzheimer's disease、Parkinson's disease、poly-glutamine disease、ALS、catalact、trigger factor、NAC、HSP70、HSP40、BAG1、HSP60、HSP10、CCT、HSP90、HSP104、small HSPs、α-crystallin、Transcriptional regulation of chaperone genes、HSF、HSE、rpoH、ERSE, ATF6, UPRE, XBP1, Hac1p, IRE1, PERK, ATF4, AARE, ER chaperone, ERAD, cytosolic splicing, proteolysis, TFE3, GASE, KLFs, PGSE, TFE3 pathway, proteoglycan pathway, mucin pathway, cholesterol pathway、Structure of the proteasome、αsubunit、βsuubunit、20S proteasome、26S proteasome、19S subunit、ubiquitin、types of the proteasome、immunoproteasome、thymoproteasome、testis-specific proteasome、chaperones specialized for the proteasome assembly、PAC1、PAC2、PAC3、deubiquitination、catalytic subunit、function of the proteasome、cell cycle、CDK（cyclin-dependent kinase）、cyclin、NF-kB、IkB、MHC、antigen presentation、ERAD、cancer、proteasome-like structures in archaea and eubacteria、bortezomib（velcade）、proteasome、HslUV、Function of ubiquitin、Structure of ubiquitin、K48 poly-ubiquitin、K63 poly-ubiquitin、M1 poly-ubiquitin、Conjugation of ubiquitin to substrates、proteasome、endocytosis、NEMOkinase、VHL、FBS1、ubiquitin-like molecules、SUMO、NEDD8、ATG12、ATG8、ISG-15、Urm1、UFM1、Function of the autophagy、isolation membrane、autophagosome、three types of the autophagy、macroautophagy、microautophagy、chaperone-mediated autophagy、molecular mechanism of the autophagy、ATG genes、TOR、ATG1、ATG13、VPS34、ATG12、ATG5、ATG8、ATG32、phosphatidylethanolamine、ATG9、HSP70、LAMP2A、p62、mitophagy、pexophagy、ERphagy、autophagy to pathogens、structure of the lysosome、V-ATPase、低pH、cathepsine、function of the lysosome、acrosome、lysosomal storage diseases、Separase、chromosome、securin、cohesin、APC complex、CDC20、caspase、apoptosis、necrosis、DNA degradation（DNA ladder）、caspase 9、caspase 8、caspase 3、death receptor、death ligand、pro-caspase、apoptosome、Apaf-1、Bax、Bak、Bcl2、cytochrome c、eat me signal、thrombin、fibrinogen、proto-thrombin、factor X、factor Xa、hemophilia、hirudin、Signal sequence、SRP and its structure、SRP receptor、translocon and its structure、Sec61、co-translational translocation、post-tranlational translocation、molecular chaperone、clathrin vesicle、COP-I vesicle、COP-II vesicle、clathrin、dynamin、rab protein、v-SNARE、t-SNARE、ER exit site、Sar1 protein、KDEL、KDEL receptor、mannose-6 phosphate、mannose-6 phosphate receptor、calnexin cycle、ERAD、Nuclear localization signal、nuclear export signal、nuclear pore、importin、Ran-GTP、Ran-GAP、Ran-GEF、exportin、NF-AT、mitochondrial targeting signal）、TOM、TIM、SAM、mitchondria、mitchondria、matrix、、mitochondrial chaperone、TOC、TIC、stroma、thyrakoid、PEX、PEX5、ubiquitination

Course Description: Understanding the function and structure of proteins does not mean that students understand proteins. In this lecture, we will explain the "proteostasis," the series of processes that occur in the life of proteins, from their birth, to their transport to the place where it functions, to their eventual death.

Lecture Methods: Students will take a short test at the beginning of the lecture to grasp the contents to be learned on the day of the lecture. The lectures are basically given in Japanese, but students will learn basic knowledge in English from time to time. Students will take a short exam at the end of the lecture to measure their level of achievement.

Lecture Plan:
(1)Introduction
Lecture 1: Life of Proteins (Proteostasis)（Lecture Resume 1）
Lecture 2: Trigger factor and NAC（Lecture Resume２）

(2) Molecular chaperones and related diseases
Lecture 3: HSP70-HSP40-GrpE（Lecture Resume３）
Lecture 4: HSP60-HSP10 and CCT（Lecture Resume４）
Lecture 5: HSP90, HSP104, small HSPs, HSF and heat shock response（Lecture Resume５）
Lecture 6: Proteasome（Lecture Resume６）
Lecture 7: Ubiquitin（Lecture Resume７）
(3) Degradation of proteins and related diseases
Lecture 8: Mini test
Lecture 9: Ubiquitin-like molecules（Lecture Resume９）
Lecture 10: Autophagy（Lecture Resume１０）
Lecture 11: Lysosome and other proteases（Lecture Resume１１）
(4) Subcellular localization of proteins and related diseases
Lecture 12: Proteins in the endoplasmic reticulum（Lecture Resume１２）
Lecture 13: Proteins in the Golgi apparatus and the lysosome（Lecture Resume１３）
Lecture 14: Proteins in the mitochondria and chloroplast（Lecture Resume１４）
Lecture 15: Proteins in the nucleus and the peroxisome（Lecture Resume１５）
Examination

In-person

・対面授業のみ
・遠隔授業単位上限の適用を受けない

Limited permission for use

生成AIの利用にあたっては『本学の教育における生成AIの取扱いについて(学生向け)』の記載内容について留意すること。

この授業においては、以下の範囲において、生成AIの利用を許可し、これ以外の範囲での利用は禁止する。生成AIの利用については担当教員の指示に従うこと。教員が認める範囲を超えて生成AIを利用し たことが判明した場合は、単位を認定しない、又は認定を取り消すこ とがある。生成AIの出力した内容について、事実関係の確認や出典・参考文献を確認・追記することが重要である。また、生成AIによる出力結果をそのまま課題・レポートとして提出してはならない。

<利用可の範囲>
課題・レポート作成の際の参考として使用すること。

Peter Walter Ed.：Essential Cell Biology, WW Norton & CO

Peter Walter Ed.：Molecular Biology of the Cell　WW Norton & CO
Kazutoshi Mori：Molecular Biology in cells　Kodansha

Not applicable

【Grading Criteria】 For those who can understand and explain "Proteostasis," credits will be awarded based on the achievement level of the abilities (knowledge, skills, thinking, judgment, expression, etc.) described in the lecture objectives and achievement goals, with grades of S (90 points or higher), A (80 points or higher), B (70 points or higher), and C (60 points or higher). 【Grading Methods】

The evaluation will be based on 50% of the mid term-examination (held in May) and 50% of the regular examination (held in August), as well as the overall evaluation including the students' attitudes (active questioning, etc.).

The quiz at the beginning of the lecture will be explained in the lecture on the same day.
　　　　　　　　　　　　
Reports assigned after the lecture will be returned via e-mail with comments for each one, and the excellent ones will be critiqued, introducing them in the next lecture.

For mid-term and regular exams, overall critiques and model answers will be given during the lecture and using the class profile function in Universal Passport.

Basic biology courses are preferred. English proficiency is not required.
Students are expected to attend lectures after sufficient preparation and review of the distributed lecture materials.

Not applicable

特になし